Filamentous microalga Klebsormidium sp.has huge potential to become a natural and healthy additive in aquatic feed since it contains various bioactive nutrients, such as linoleic acid (LA), carotenoids, and chlorophylls.Therefore, an eight-week feeding experiment was performed to Anodizing Service evaluate the effects of dietary Klebsormidium sp.on the growth performance, antioxidant and anti-inflammatory status, metabolism, and mid-intestine morphology of Litopenaeus vannamei.Two isonitrogenous and isolipid diets supplemented with and without 5% Klebsormidium sp.
were prepared.Results showed that L.vannamei fed with Klebsormidium sp.had better growth performance and feed utilization by optimizing mid-intestine morphology and improving the carbohydrate metabolism.In addition, Klebsormidium sp.
also enhanced the antioxidant capacity of L.vannamei by downregulating antioxidant parameters (hepatopancreas T-SOD, hepatopancreas GSH-PX, hemolymph T-SOD, hemolymph MDA) and RNA expression levels of antioxidant genes (gsh-px and cat).Furthermore, the supplementations of dietary Klebsormidium sp.significantly improved hepatopancreas health by downregulating RNA expression levels of pro-inflammatory related genes (relish and rho).Therefore, a dose of Doors 5% Klebsormidium sp.
is recommended for the daily diet of L.vannamei to improve the growth performance, antioxidant and anti-inflammatory status, metabolism, and mid-intestine morphology of shrimp.